BI3B67-Microbiology Field Course

Module Provider: School of Biological Sciences
Number of credits: 20 [10 ECTS credits]
Level:6
Terms in which taught: Summer term module
Pre-requisites: BI1S1 Introductory Microbiology
Non-modular pre-requisites:
Co-requisites:
Modules excluded:
Current from: 2023/4

Module Convenor: Dr Glyn Barrett
Email: glyn.barrett@reading.ac.uk

Type of module:

Summary module description:

A unique opportunity to travel to and study microbial biodiversity, survival mechanisms and biotechnological applications of extremophiles in Northern Iceland or Colombia (alternate years). Dependent on destination, you will be sampling from geothermal pools, glaciers and volcano slopes or lush tropical rainforest soils. Microbes will be cultured, identified and characterised in world class laboratories in destination countries where you will be working in multicultural teams of students.

Aims:

The aim of this module is to provide students with an opportunity to learn field-based sampling techniques and to learn about the microbial biodiversity in the Arctic and tropics and how it is changing. This will be done by teaching the students sampling and analysis techniques. The practical aspect will facilitate a better understanding of science outside the laboratory, but it will also improve students’ learning of theory on microbial evolution and function, particularly those of extremophiles. Therefore, the project will be a novel and exciting addition to teaching and learning. Moreover, we aim to make the field trip a joint module with Icelandic students, Colombian and American students, so that our hosts at UnAk or EAFIT will partner us in teaching.

Assessable learning outcomes:

By the end of the module it is expected that the student will be able to:

• Design an appropriate approach for sampling from different environments


• Implement a range of culturing techniques to isolate different microorganisms


• Identify different characteristics of extremophiles


• Identify and implement analytical techniques including genomics for characterisation of extremophiles


• Understand the roles o f microbes in various environments and how the environment shapes bacterial evolution


• Understand the microbial characteristics that enable survival in extreme conditions and potential biotechnological applications

Additional outcomes:

• Interaction with other EU/South American/North American students and life in an Icelandic/Colombian university, including innovative teaching methods such as distance learning

Outline content:

The field trip will be preceded by seminars to discuss the outline of the trip and to aid the students in designing the approaches they will take for sampling and analyses. In Iceland/Colombia, the students will spend 3-4 days sampling, with examples described below.

1. Extremophile analysis: sampling from geothermal pools and volcano slopes.


2. Glacial microbial biodiversity: water, glacier and lichen sampling.


3. Tropical microbial bio diversity: jungle and banana plantation.


4. River/mountain/cave/desert sampling to analyse microbial biodiversity from different extreme habitats.

Once the samples have been acquired, the students will then use the laboratory facilities at UnAk/EAFIT to culture the microbes and carry out different techniques to characterise the diversity and the microbes, these are detailed below.

• Culturing bacteria and extremophiles


• Plating bacteria for most probable number analysis using indicator dyes


• Use tests to analyse for SRB and NRBs


• Multiplex PCR for group-specific analysis


• Microscopy to analyse extremophile cells


• Examination of biocontrol properties

Concerning the 5-6 day period in the laboratory, we are aiming to give a lecture at the beginning of the day and a seminar at the end of the day, with practical work in the middle. In Iceland, we aim to obtain lectures from members of the Arctic council in Iceland and Canada at other institutions, by a distance learning link, as well as lectures from people at UnAk and other institutions in Iceland. In Colombia, we aim to obtain lectures from members of EAFIT, Universidad de Antioquia, Reading, Akureyri and University of Massachusetts.

Global context:

Interaction with other EU/South American/North American students and life in an Icelandic/Colombian university, including innovative teaching methods such as distance learning.

Brief description of teaching and learning methods:

There will be pre-trip discussion sessions, field trips to take samples and record environmental data, plus several sessions in the laboratory to identify and analyse microbes. Informal advice from the supervisors will be available throughout the data collection. The data from the field trip will be compiled and the data analysed. A symposium day will be held whereby students are grouped into teams and make a presentation of their results (a free day will be provided the day before this to al low them to do this). The results from their field and lab work will be recorded in a lab book, which will be assessed daily. A lay blog will be written and submitted for the first day ofÌýAutumn term. The lab book and a dissertation, including background introduction, materials and methods, results and discussion, will be written and submitted by the middle of Autumn term.

Contact hours:

Ìý	Autumn	Spring	Summer
Lectures			10
Tutorials			10
Practicals classes and workshops			30
Fieldwork			30
Guided independent study:	Ìý	Ìý	Ìý
Ìý Ìý Wider reading (independent)			10
Ìý Ìý Preparation for presentations			10
Ìý Ìý Group study tasks			10
Ìý Ìý Carry-out research project			20
Ìý Ìý Dissertation writing			40
Ìý Ìý Essay preparation			30
Ìý	Ìý	Ìý	Ìý
Total hours by term	0	0	200
Ìý	Ìý	Ìý	Ìý
Total hours for module	200

Summative Assessment Methods:

Method	Percentage
Dissertation	70
Oral assessment and presentation	30

Summative assessment- Examinations:

Summative assessment- Coursework and in-class tests:

30% group presentation

70% dissertation

Formative assessment methods:

Penalties for late submission:

The Support Centres will apply the following penalties for work submitted late:

• where the piece of work is submitted after the original deadline (or any formally agreed extension to the deadline): 10% of the total marks available for that piece of work will be deducted from the mark for each working day (or part thereof) following the deadline up to a total of five working days;
• where the piece of work is submitted more than five working days after the original deadline (or any formally agreed extension to the deadline): a mark of zero will be recorded.

The University policy statement on penalties for late submission can be found at: /cqsd/-/media/project/functions/cqsd/documents/cqsd-old-site-documents/penaltiesforlatesubmission.pdf
You are strongly advised to ensure that coursework is submitted by the relevant deadline. You should note that it is advisable to submit work in an unfinished state rather than to fail to submit any work.

Assessment requirements for a pass:
A mark of 40% overall

Reassessment arrangements:

Resubmission of dissertation.

Additional Costs (specified where applicable):

1. Required text books:ÌýMany e-books freely available at the Library


2. Specialist equipment or materials:


3. Specialist clothing, footwear or headgear:ÌýHiking boots, warm clothes


4. Printing and binding:


5. Computers and devices with a particular specification:


6. Travel, accommodation and subsistence:

Last updated: 13 April 2023